概述

  • 产品名称Anti-BRAP抗体
    参阅全部 BRAP 一抗
  • 描述
    兔多克隆抗体to BRAP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 BRAP.

    (Peptide available as ab88155.)

  • 阳性对照
    • This antibody gave a positive signal in MCF7 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab77721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
ICC/IF 1/200. Use with paraformaldehyde fixed cells.

靶标

  • 相关性BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • 3010002G07Rik antibody
    • BRAP2 antibody
    • BRCA1 associated protein antibody
    • EC 6.3.2. antibody
    • Galectin 2 binding protein antibody
    • IMP antibody
    • Impedes mitogenic signal propagation antibody
    • Renal carcinoma antigen NY REN 63 antibody
    • RING finger protein 52 antibody
    • RNF52 antibody
    • zgc:92894 antibody
    see all

Anti-BRAP antibody 图像

  • Anti-BRAP antibody (ab77721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • ab77721 (1/200) staining BRAP in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

Anti-BRAP antibody (ab77721)参考文献

ab77721 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"