Branched Chain Amino Acid Assay试剂盒(ab83374)

概述

  • 产品名称Branched Chain Amino Acid Assay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • 检测类型Quantitative
  • 灵敏度
    > 0.2 nmol/well
  • 检测时间
    0h 40m
  • 产品概述

    Abcam's Branched Chain Amino Acid Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (?max = 450 nm). Abcam's Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects.
  • 别名
    • BCAA
    • Isoleucine
    • Leucine
    • Valine

应用

Our Abpromise guarantee covers the use of ab83374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Branched Chain Amino Acid Assay Kit 图像

  • BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).

  • BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).

  • Leucine Standard Curve performed according to the attached protocol.

实验方案

Branched Chain Amino Acid Assay Kit (ab83374)参考文献

This product has been referenced in:
  • Serralde-Zúñiga AE  et al. Omental adipose tissue gene expression, gene variants, branched-chain amino acids, and their relationship with metabolic syndrome and insulin resistance in humans. Genes Nutr 9:431 (2014). Read more (PubMed: 25260659) »

See 1 Publication for this product

Product Wall

The plasma samples can be diluted with water and then after adding to the well, the volume must be filled up to 50 ul with assay buffer.


ab83374 (Branched Chain Amino Acid Assay Kit). We have not tested this kit with cell or tissue lysates yet but only with fluids like Cell culture supernatant, Urine, Serum, Plasma and can therefore not provide more than a general protocol below. Read More

Thank you for contacting us.

This kit will detect all branched chain amino acids, regardless of whether they are single amino acids or peptides.

I hope this information is helpful to you. Please do not hesitate to contact us if you ne...

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Merci de nous avoir contactés.

Le laboratoire m'a confirmé que nous n'avons malheureusement pas de données expérimentales pour ces kits sur des lysats musculaires.

Désolé de ne pas pouvoir vou...

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Thank you for your inquiry.

I am happy to confirm that this kit will measure all the Branched chain amino acids (BCAAs).

All BCAAs will be deaminated to produce NADH, which is what this kit will actually measure.
The Leucine in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"