Brain: cerebrum (大鼠)组织裂解液(ab4033)

概述

  • 产品名称Brain: cerebrum (大鼠)组织裂解液
  • 常规说明Source: Outbred Sprague Dawley Albino rat.


    Rat cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明Rat cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab4033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent concentration. Ready to load; 10-20 µg/lane is recommended for mini gel.

    Brain: cerebrum (Rat) Tissue Lysate (ab4033)参考文献

    ab4033 has not yet been referenced specifically in any publications.

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