Anti-Brachyury / Bry抗体(ab57480)

概述

  • 产品名称Anti-Brachyury / Bry抗体
    参阅全部 Brachyury / Bry 一抗
  • 描述
    小鼠单克隆抗体to Brachyury / Bry
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL SMLQSHDNW, corresponding to amino acids 222-321 of Human Brachyury/ Bry

性能

应用

Our Abpromise guarantee covers the use of ab57480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • 疾病相关Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • 序列相似性Contains 1 T-box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Anti-Brachyury / Bry antibody 图像

  • Anti-Brachyury / Bry antibody (ab57480) at 1 µg + A-549 cell lysate at 25 µg

    Predicted band size : 47 kDa

Anti-Brachyury / Bry antibody (ab57480)参考文献

This product has been referenced in:
  • Palena C  et al. Overexpression of the EMT driver brachyury in breast carcinomas: association with poor prognosis. J Natl Cancer Inst 106:N/A (2014). Human . Read more (PubMed: 24815864) »
  • Jezkova J  et al. Brachyury regulates proliferation of cancer cells via a p27Kip1-dependent pathway. Oncotarget 5:3813-22 (2014). IHC . Read more (PubMed: 25003467) »

See all 4 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Stem Cells)
Specification Stem Cells
Fixative Formalin
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
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Verified customer

提交于 Jan 26 2012

Thank you for contacting us and reporting the problems your customer has been experiencing with anti-Brachyury / Bry antibody (ab57480).

We take product complaints very seriously, and investigate every product that we feel may not be perform...

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DISCOUNT CODE: xxxxxx Expiration date: 2012-04-02 I am very pleased to hear you would like to test ab57480 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"