Anti-Brachyury / Bry抗体(ab20680)

概述

  • 产品名称Anti-Brachyury / Bry抗体
    参阅全部 Brachyury / Bry 一抗
  • 描述
    兔多克隆抗体to Brachyury / Bry
  • 经测试应用适用于: ICC/IF, IHC-Fr, Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Dog不与反应: Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Brachyury.

    (Peptide available as ab21992.)

  • 阳性对照
    • ab20680 gives a positive signal in Murine Embryonic Stem Cells differentiated to express mesoderm markers in WB and Human brain tissue sections by Immunohistochemistry .

性能

应用

Our Abpromise guarantee covers the use of ab20680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 21083428ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • 疾病相关Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • 序列相似性Contains 1 T-box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Anti-Brachyury / Bry antibody 图像

  • A proportion of nuclei from Day 3 Mouse EBs (derived from R1 cells) stained positive for Brachury using ab20680. In Day 8 EBs a vastly reduced proportion of nuclei stained positive (not shown). In day 10 EBs no nuclei stained Brachyury-positive. The IHC-P data for Brachyury in EBs corresponded with qRT-PCR expression data (not shown). Controls on Day 3 EBs in which primary or secondary antibody were omitted, undifferentiated ES cells and feeder cells (MEFs) all exhibited very little background staining. Zinc was used for tissue fixation.
  • Anti-Brachyury antibody, ab20680, stained nuclei of human embryonic stem cells differentiated into mesendoderm. As would be expected, mesoderm cells express Brachyury whereas endoderm cells are negative.
  • Anti-Brachyury / Bry antibody (ab20680) at 1 µg/ml + Whole Cell Lysate from Murine ES Cells Differentiated to Express Mesoderm Markers at 15 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
    ab20680 recognizes a band of 53 kDa in Murine Embryonic Stem Cells differentiated to express mesoderm markers. This corresponds in size to the Brachyury protein which has a predicted molecular weight of 47 kDa. A band of 75 kDa has also been observed, although we are unsure as to the identity of this band.
  • ab20680 staining Brachyury in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/2000 in horse serum) for 12 hours. A Streptavidin-conjugated Horse anti-rabbit monoclonal (1/250) was used as the secondary antibody.

    See Abreview

Anti-Brachyury / Bry antibody (ab20680)参考文献

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »
  • Neri T  et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports 5:558-68 (2015). ICC/IF ; Mouse . Read more (PubMed: 26344905) »

See all 15 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, EDTA, none
Sample Zebrafish Tissue sections (Sagittal sections of whole formalin fixed, decalci)
Specification Sagittal sections of whole formalin fixed, decalci
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Treatment Differentiating Conditions (Minus LIF Medium)
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

提交于 Dec 28 2012

Thank you for contacting us.



A. has forwarded your email to us in Scientific Support, and I would like to know what kind of application you are using?


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (6.5 embryo)
Loading amount 5 µg
Specification 6.5 embryo
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Jim Manavis

Verified customer

提交于 Oct 22 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryoid bodies)
Specification Embryoid bodies
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - triton-x100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"