Mouse, Rat, Rabbit, Cow
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-216 of Human BPGM (NP_001715).
- 293T, A431 and H1299 whole cell lysates; HeLa cells; HeLaS3, Molt-4 and Raji cell lines
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 30 kDa.
||1/100 - 1/200.
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 126.96.36.199) and phosphatase (EC 188.8.131.52) activities.
Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis.
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
Glycation of Lys-159 in diabetic patients inactivates the enzyme.
Information by UniProt
- 2 antibody
- 2,3-bisphosphoglycerate mutase antibody
- 2,3-bisphosphoglycerate mutase, erythrocyte antibody
Western blot - BPGM antibody (ab97497)
All lanes : Anti-BPGM antibody (ab97497) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - BPGM antibody (ab97497)
ab97497, at 1/100 dilution, staining BPGM in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
has not yet been referenced specifically in any publications.
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