Anti-BPGM抗体(ab97497)
Key features and details
- Rabbit polyclonal to BPGM
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BPGM抗体
参阅全部 BPGM 一抗 -
描述
兔多克隆抗体to BPGM -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow -
免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-216 of Human BPGM (NP_001715).
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阳性对照
- 293T, A431 and H1299 whole cell lysates; HeLa cells; HeLaS3, Molt-4 and Raji cell lines
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. -
疾病相关
Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. -
序列相似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. -
翻译后修饰
Glycation of Lys-159 in diabetic patients inactivates the enzyme. - Information by UniProt
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数据库链接
- Entrez Gene: 669 Human
- Entrez Gene: 12183 Mouse
- Entrez Gene: 100009096 Rabbit
- Entrez Gene: 296973 Rat
- Omim: 613896 Human
- SwissProt: P07738 Human
- SwissProt: P15327 Mouse
- SwissProt: P07952 Rabbit
see all -
别名
- 2 antibody
- 2,3-bisphosphoglycerate mutase antibody
- 2,3-bisphosphoglycerate mutase, erythrocyte antibody
see all
图片
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All lanes : Anti-BPGM antibody (ab97497) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
12% SDS-PAGE -
ab97497, at 1/100 dilution, staining BPGM in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97497 被引用在 2 文献中.
- Yin GQ et al. Differential proteomic analysis of children infected with respiratory syncytial virus. Braz J Med Biol Res 54:e9850 (2021). PubMed: 33656056
- Xu G et al. Bisphosphoglycerate Mutase Deficiency Protects against Cerebral Malaria and Severe Malaria-Induced Anemia. Cell Rep 32:108170 (2020). PubMed: 32966787