Anti-Bovine Serum Albumin抗体[5F9] (ab9092)

概述

  • 产品名称Anti-Bovine Serum Albumin抗体[5F9]
    参阅全部 Bovine Serum Albumin 一抗
  • 描述
    小鼠单克隆抗体[5F9] to Bovine Serum Albumin
  • 特异性This antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Cow
    不与反应: Chicken, Human
  • 常规说明This product contains 0.02% (w/v) Sodium Azide preservative.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号5F9
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明This product was assayed by immunoblot using IRDye800TM Goat-anti-Mouse IgG.
    A working dilution of 1:5,000 is suggested for this assay.
    Researchers should determine optimal titers for other applications.
    The antibody may be suitable for other applications, although no supporting data is available
    ELISA: Use at an assay dependent dilution.
  • 靶标

    • 相关性Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
    • 细胞定位Secreted
    • 数据库链接
    • 别名
      • ALB antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • BSA antibody
      • Cell growth inhibiting protein 42 antibody
      • Growth inhibiting protein 20 antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    Anti-Bovine Serum Albumin antibody [5F9] 图像

    • Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.

      Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.

    Anti-Bovine Serum Albumin antibody [5F9] (ab9092)参考文献

    This product has been referenced in:
    • Li X  et al. Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran. Colloids Surf B Biointerfaces 61:113-7 (2008). Read more (PubMed: 17703925) »

    See 1 Publication for this product

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    Please type Bovine Serum Album in the search box or click on the following link

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    I hope this information is helpful to you. Please do n...

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    Ab9092 was purified from ascites.

    Thank you for your enquiry. We would like to confirm that this antibody derived from ascites.

    Albumin is the most abundant protein in serum. I don't know what dilutions you did to human serum? The antibody would non-specifically bind to HSA when HSA overwhelmingly presented in the loading sample. I suggest you try to dilute both sera at least 1...

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    Thank you for your message. We would suggest trying PBS with 0.1% Tween 20 and 1.5% fish gel as blocking and dilution buffer. Load 20uL of purified BSA at 0.5mg/mL and use 1:1000 dilution of the primary antibody.

    For the protocol please reference our ELISA under Methods on the web. The BSA we've been using is Fraction V (IgG and Protease free).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"