Anti-Bovine Papilloma Virus E2抗体[5H4] (ab1072)

概述

性能

应用

Our Abpromise guarantee covers the use of ab1072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • 细胞定位Host cell nucleus
    • 别名
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    Anti-Bovine Papilloma Virus E2 antibody [5H4] 图像

    • Immunofluorescent staining of U2OS cells expressing BPV E2 protein with ab1072. The cells were counter-stained with DAPI, and the upper shows an image taken with illumination at both 460 nm and 488 nm, whereas the lower image shows illumination at 488 nm.

    Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)参考文献

    ab1072 has not yet been referenced specifically in any publications.

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    I'm sorry to hear you are having problems with ab1072. I am currently trying to find out more information about the protocol used to test the antibody in WB. Have you tested the blot without milk blocking and if the non specific bands could be due ...

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