Anti-Bovine Papilloma Virus E2抗体[1E4] (ab980)

概述

  • 产品名称Anti-Bovine Papilloma Virus E2抗体[1E4]
    参阅全部 Bovine Papilloma Virus E2 一抗
  • 描述
    小鼠单克隆抗体[1E4] to Bovine Papilloma Virus E2
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Cow
  • 免疫原

    Mapped to amino acids 250-280.

  • 表位Not Determined.

性能

应用

Our Abpromise guarantee covers the use of ab980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • 细胞定位Host cell nucleus
    • 别名
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    Anti-Bovine Papilloma Virus E2 antibody [1E4] 图像

    • All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

      Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
      Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
      Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
      Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg


      Predicted band size : 45 kDa

    Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)参考文献

    This product has been referenced in:
    • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). Read more (PubMed: 10233926) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"