概述

  • 产品名称Anti-BOULE抗体
    参阅全部 BOULE 一抗
  • 描述
    山羊多克隆抗体to BOULE
  • 经测试应用适用于: ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    (C)QPEPIKTVWSIHY

    , corresponding to C terminal amino acids 271-283 of Human BOULE

  • 阳性对照
    • IHC-P: FFPE human testis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 4 - 6 µg/ml.

靶标

  • 功能Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • 组织特异性Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis.
  • 序列相似性Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bol (Drosophila boule homolog) like antibody
    • Bol antibody
    • Bol boule like antibody
    • Bol, boule like (Drosophila) antibody
    • bol, boule-like (Drosophila) antibody
    • Boll antibody
    • BOLL_HUMAN antibody
    • BOULE antibody
    • Boule like antibody
    • BOULE, Drosophila, homolog of antibody
    • Protein boule like antibody
    • Protein boule-like antibody
    • Putative uncharacterized protein BOLL antibody
    see all

Anti-BOULE antibody 图像

  • IHC image of BOULE staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28745, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. ab28745 (4µg/ml) staining following steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-BOULE antibody (ab28745)参考文献

ab28745 has not yet been referenced specifically in any publications.

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