Anti-Borealin/CDCA8抗体(ab74473)
Key features and details
- Rabbit polyclonal to Borealin/CDCA8
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Borealin/CDCA8抗体
参阅全部 Borealin/CDCA8 一抗 -
描述
兔多克隆抗体to Borealin/CDCA8 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Rat kidney tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa). |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
序列相似性
Belongs to the borealin family. -
发展阶段
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
结构域
The C-terminal region (aa 207-280) represents the dimerization motif. -
翻译后修饰
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
细胞定位
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
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数据库链接
- Entrez Gene: 500545 Rat
- SwissProt: Q6AXW0 Rat
- Unigene: 14970 Rat
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别名
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
see all
图片
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Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml
All lanes : Rat kidney lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab74473 被引用在 1 文献中.
- Kim HJ et al. Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells. Nucleic Acids Res 46:6544-6560 (2018). PubMed: 29901724