概述

  • 产品名称Anti-Borealin/CDCA8抗体
    参阅全部 Borealin/CDCA8 一抗
  • 描述
    兔多克隆抗体to Borealin/CDCA8
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the center region of human Borealin/CDCA8

  • 阳性对照
    • HL60 cytoplasmic or nuclear lysates; Paraffin embedded Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab70910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 序列相似性Belongs to the borealin family.
  • 发展阶段Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • 结构域The C-terminal region (aa 207-280) represents the dimerization motif.
  • 翻译后修饰Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • 细胞定位Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Anti-Borealin/CDCA8 antibody 图像

  • Anti-Borealin/CDCA8 antibody (ab70910) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 18 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab70910, at a 1/10 dilution, staining human Borealin/CDCA8 in lung carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70910 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-Borealin/CDCA8 antibody (ab70910)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% Triton in PBS
Specification HeLa
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Apr 06 2016

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