概述

  • 产品名称
    Anti-Borealin/CDCA8抗体
    参阅全部 Borealin/CDCA8 一抗
  • 描述
    小鼠多克隆抗体to Borealin/CDCA8
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native Borealin/CDCA8 (purified) (Human)

  • 阳性对照
    • Borealin/CDCA8 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent dilution. PFA fixation is necessary

靶标

  • 功能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 序列相似性
    Belongs to the borealin family.
  • 发展阶段
    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • 结构域
    The C-terminal region (aa 207-280) represents the dimerization motif.
  • 翻译后修饰
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • 细胞定位
    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

图片

  • All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution

    Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • ab67126 staining Borealin/CDCA8 in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 10% BSA for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/300 dilution.
    When fixed in PFA, the antibody stains the centromere in metaphase and the midzone in anaphase, as expected for Borealin, a CPC protein. Borealin (red), Kinetochores (green), DAPI (blue).

    See Abreview

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
15 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 19 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Cell lysate - whole cell (DF1)
Loading amount
15 µg
Specification
DF1
Gel Running Conditions
Reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa (Human cervical cancer cells))
Specification
HeLa (Human cervical cancer cells)
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0.1% Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 25 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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