产品名称 Anti-BOP1抗体 参阅全部 BOP1 一抗
WB, IP more details
Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).
HeLa and 293T whole cell lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
Use at 2-5 µg/mg of lysate.
功能 Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
序列相似性 Belongs to the WD repeat BOP1/ERB1 family. Contains 7 WD repeats.
细胞定位 Nucleus > nucleolus. Nucleus > nucleoplasm.
Information by UniProt
Block of proliferation 1 antibody
Anti-BOP1 antibody 图像
Western blot - BOP1 antibody (ab86653)
All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 84 kDa Observed band size : 100,110 kDa ( why is the actual band size different from the predicted?) Additional bands at : 120 kDa,65 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - BOP1 antibody (ab86653)
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 90 seconds
Anti-BOP1 antibody (ab86653)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"