Anti-BOP1抗体(ab86653)
Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BOP1抗体
参阅全部 BOP1 一抗 -
描述
兔多克隆抗体to BOP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86653于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
序列相似性
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23246 Human
- Omim: 610596 Human
- SwissProt: Q14137 Human
- Unigene: 645279 Human
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别名
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
see all
图片
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All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 84 kDa
Observed band size: 100,110 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 65 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 90 seconds -
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 90 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab86653 尚未被引用在任何文献中。