概述

  • 产品名称
  • 描述
    兔多克隆抗体to Bok
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the N-terminal region (BH3 domain) of human Bok.

  • 阳性对照
    • HL-60 cell lysate; Parraffin embedded human hepatocarcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab71646 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

靶标

图片

  • Anti-Bok antibody (ab71646) at 1/100 dilution + HL-60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab71646 at 1/50 dilution, staining Bok in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

文献

ab71646 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hepatocytes and Bone marrow cells)
Loading amount
50 µg
Specification
Hepatocytes and Bone marrow cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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