Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 134-163 of Human Bod1
MCF-7 cell line lysates
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 19 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.
Belongs to the BOD1 family.
Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase.
Information by UniProt
Biorientation defective 1 antibody
Biorientation defective protein 1 antibody
biorientation of chromosomes in cell division 1 antibody
Western blot - Anti-Bod1 antibody (ab103510)
Anti-Bod1 antibody (ab103510) at 1/100 dilution + MCF-7 cell line lysates at 35 µg
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bod1 antibody (ab103510)
IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.ab103510 used at 1:50 dilution.
has not yet been referenced specifically in any publications.
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