RabMAb

Anti-BOB1抗体[EP3300] (ab76010)

概述

  • 产品名称Anti-BOB1抗体[EP3300]
    参阅全部 BOB1 一抗
  • 描述
    兔单克隆抗体[EP3300] to BOB1
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in human BOB1.

  • 阳性对照
    • Ramos cell lysate. Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
  • 应用说明ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/5000 - 1/30000. Predicted molecular weight: 28 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
    • 组织特异性B-cell specific.
    • 疾病相关Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
    • 序列相似性Belongs to the POU2AF1 family.
    • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B cell Oct binding protein 1 antibody
      • B cell specific coactivator OBF 1 antibody
      • B cell specific coactivator OBF1 antibody
      • B-cell-specific coactivator OBF-1 antibody
      • BOB 1 antibody
      • BOB-1 antibody
      • OBF 1 antibody
      • OBF1 antibody
      • OBF1_HUMAN antibody
      • OCA B antibody
      • OCA-B antibody
      • OCAB antibody
      • OCT binding factor 1 antibody
      • OCT-binding factor 1 antibody
      • POU class 2 associating factor 1 antibody
      • POU domain class 2 associating factor 1 antibody
      • POU domain class 2-associating factor 1 antibody
      • Pou2af1 antibody
      see all

    Anti-BOB1 antibody [EP3300] 图像

    • Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-BOB1 antibody [EP3300] (ab76010)参考文献

    ab76010 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"