概述

  • 产品名称Anti-BOB1抗体
    参阅全部 BOB1 一抗
  • 描述
    山羊多克隆抗体to BOB1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    YTEYVPHEAVSCP

    , corresponding to internal sequence amino acids 105-117 of Human BOB1 (NP_006226.2).

  • 阳性对照
    • HEK293 transiently expressing full-length Human BOB1 (myc and DYKDDDDK tagged).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab118389 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
  • 组织特异性B-cell specific.
  • 疾病相关Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • 序列相似性Belongs to the POU2AF1 family.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell Oct binding protein 1 antibody
    • B cell specific coactivator OBF 1 antibody
    • B cell specific coactivator OBF1 antibody
    • B-cell-specific coactivator OBF-1 antibody
    • BOB 1 antibody
    • BOB-1 antibody
    • OBF 1 antibody
    • OBF1 antibody
    • OBF1_HUMAN antibody
    • OCA B antibody
    • OCA-B antibody
    • OCAB antibody
    • OCT binding factor 1 antibody
    • OCT-binding factor 1 antibody
    • POU class 2 associating factor 1 antibody
    • POU domain class 2 associating factor 1 antibody
    • POU domain class 2-associating factor 1 antibody
    • Pou2af1 antibody
    see all

Anti-BOB1 antibody 图像

  • Lane 1 : Anti-BOB1 antibody (ab118389) at 0.5 µg/ml
    Lane 2 : Anti-BOB1 antibody (ab118389) at 1 mg/ml
    Lane 3 : Anti-DYKDDDDK Tag antibody at 1/3000 dilution

    Lane 1 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged)
    Lane 2 : HEK293 cell lyaste, mock transfected
    Lane 3 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged)

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 27 kDa

Anti-BOB1 antibody (ab118389)参考文献

ab118389 has not yet been referenced specifically in any publications.

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