The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 27 kDa).
功能Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
疾病相关Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
序列相似性Belongs to the POU2AF1 family.
翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Lane 1 : Anti-BOB1 antibody (ab118389) at 0.5 µg/ml Lane 2 : Anti-BOB1 antibody (ab118389) at 1 mg/ml Lane 3 : Anti-DYKDDDDK Tag antibody at 1/3000 dilution
Lane 1 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged) Lane 2 : HEK293 cell lyaste, mock transfected Lane 3 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged)
Lysates/proteins at 10 µg per lane.
Predicted band size : 27 kDa
Anti-BOB1 antibody (ab118389)参考文献
has not yet been referenced specifically in any publications.