重组Anti-BNIP3L/NIX (phospho S81)抗体[EPR19826] (ab208190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19826] to BNIP3L/NIX (phospho S81)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BNIP3L/NIX (phospho S81)抗体[EPR19826]
参阅全部 BNIP3L/NIX 一抗 -
描述
兔单克隆抗体[EPR19826] to BNIP3L/NIX (phospho S81) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- Dot: BNIP3L/NIX (phosphor S81) peptide. WB: HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19826 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot |
1/1000.
|
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WB | (1) |
1/5000. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 24 kDa).
|
说明 |
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Dot blot
1/1000. |
WB
1/5000. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3. -
序列相似性
Belongs to the NIP3 family. -
翻译后修饰
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. -
细胞定位
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Omim: 605368 Human
- SwissProt: O60238 Human
- SwissProt: Q9Z2F7 Mouse
- Unigene: 131226 Human
- Unigene: 29820 Mouse
- Unigene: 347081 Mouse
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别名
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
图片
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Dot blot analysis of BNIP3L/NIX (phospho S81) labeled with ab208190 at 1/1000 dilution.
Lane 1: BNIP3L/NIX (phospho S81) peptide;
Lane 2: BNIP3L/NIX non-phospho peptide;
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (ab208190) at 1/5000 dilution
Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate
Lane 2 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, whole cell lysate
Lane 3 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, lysate then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX S81A mutant expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 40, 80 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 10467396).
The plasmids were kindly provided by our collaborator Dr. Zhong Chen, Zhejiang University.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab208190 被引用在 2 文献中.
- Zeng C et al. Cyclovirobuxine D Induced-Mitophagy through the p65/BNIP3/LC3 Axis Potentiates Its Apoptosis-Inducing Effects in Lung Cancer Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34072333
- Yuan Y et al. BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2. Autophagy N/A:1-13 (2017). PubMed: 28820284