概述

  • 产品名称
  • 描述
    兔多克隆抗体to BNIP3
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide, selected from within aa 1-100 (BH3 domain) of human NIP3.

  • 阳性对照
    • Mouse brain tissue lysate and Ramos cell lysate, breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab38621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能
    Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
  • 序列相似性
    Belongs to the NIP3 family.
  • 细胞定位
    Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
    • BNIP 3 antibody
    • BNIP3 antibody
    • BNIP3_HUMAN antibody
    • NIP 3 antibody
    • NIP3 antibody
    see all

图片

  • All lanes : Anti-BNIP3 antibody (ab38621) at 1/100 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Mouse brain tissue lysate


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 15 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • This image shows human breast carcinoma stained with ab38621 at 1/50 dilution.
  • This image shows human colon carcinoma stained with ab38621 at 5ug/200ul dilution.
  • ICC/IF image of ab38621 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38621, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Cui J  et al. Rapamycin protects against gentamicin-induced acute kidney injury via autophagy in mini-pig models. Sci Rep 5:11256 (2015). WB . Read more (PubMed: 26052900) »
  • Woldt E  et al. Rev-erb-a modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy. Nat Med 19:1039-46 (2013). WB . Read more (PubMed: 23852339) »

See all 8 Publications for this product

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