Anti-BMX (phospho Y566)抗体(ab59409)

概述

  • 产品名称Anti-BMX (phospho Y566)抗体
    参阅全部 BMX 一抗
  • 描述
    兔多克隆抗体to BMX (phospho Y566)
  • 特异性This antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).

  • 阳性对照
    • Human thyroid gland tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).

靶标

  • 功能Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
  • 组织特异性Preferentially expressed in epithelial and endothelial cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 结构域SH2 domain mediates interaction with RUFY1.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bmx antibody
    • BMX non receptor tyrosine kinase antibody
    • BMX_HUMAN antibody
    • BONE MARROW KINASE, X-LINKED antibody
    • Bone marrow tyrosine kinase gene in chromosome X protein antibody
    • Cytoplasmic tyrosine protein kinase BMX antibody
    • Cytoplasmic tyrosine-protein kinase BMX antibody
    • Epithelial and endothelial tyrosine kinase antibody
    • ETK antibody
    • NKT38 antibody
    • NTK 38 antibody
    • NTK38 antibody
    • Protein tyrosine kinase BMX antibody
    • PSCTK 2 antibody
    • PSCTK 3 antibody
    • PSCTK2 antibody
    • PSCTK3 antibody
    see all

Anti-BMX (phospho Y566) antibody 图像

  • Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.
  • Anti-BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 78 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-BMX (phospho Y566) antibody (ab59409)

    Lane 1 : Extracts from HeLa cells treated with Serum 20% 15'
    Lane 2 : Extracts from HeLa cells treated with Serum 20% 15' and with the synthesized peptide.


    Predicted band size : 78 kDa

Anti-BMX (phospho Y566) antibody (ab59409)参考文献

This product has been referenced in:
  • Guryanova OA  et al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21481791) »

See 1 Publication for this product

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Ich habe nun aus dem Labor die Protokolle sowohl für die Behandlung der HeLa Zellen als auch das WB Protokoll für ab59409 bekommen.

Ich kopiere ihnen beide Protokolle unten in ...

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