概述

  • 产品名称Anti-BMPR1A抗体
    参阅全部 BMPR1A 一抗
  • 描述
    兔多克隆抗体to BMPR1A
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (10-30 aa in length) from the N-terminus of human BMPR1A, conjugated to KLH.

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
  • 组织特异性Highly expressed in skeletal muscle.
  • 疾病相关Juvenile polyposis syndrome
    Polyposis syndrome, mixed hereditary 2
    A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 10q23del antibody
    • Activin A receptor type II like kinase 3 antibody
    • Activin receptor like kinase 3 antibody
    • Activin receptor-like kinase 3 antibody
    • ACVRLK 3 antibody
    • ACVRLK3 antibody
    • ALK 3 antibody
    • ALK-3 antibody
    • ALK3 antibody
    • BMP type-1A receptor antibody
    • BMPR 1A antibody
    • Bmpr antibody
    • BMPR-1A antibody
    • Bmpr1a antibody
    • BMR1A_HUMAN antibody
    • Bone morphogenetic protein receptor type IA antibody
    • Bone morphogenetic protein receptor type IA precursor antibody
    • Bone morphogenetic protein receptor type-1A antibody
    • BR 1a antibody
    • BR1a antibody
    • CD 292 antibody
    • CD292 antibody
    • CD292 antigen antibody
    • EC 2.7.11.30 antibody
    • Serine threonine protein kinase receptor R5 antibody
    • Serine threonine protein kinase receptor R5 precursor antibody
    • Serine/threonine-protein kinase receptor R5 antibody
    • SKR 5 antibody
    • SKR5 antibody
    • zBMPR IA antibody
    • zBMPRIA antibody
    see all

Anti-BMPR1A antibody 图像

  • ab59945 at 1/50 - 1/100 dilution, staining BMPR1A in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
  • Anti-BMPR1A antibody (ab59945) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Anti-BMPR1A antibody (ab59945) at 1/1000 dilution + Cultured Human lymphoblast whole cell lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-BMPR1A antibody (ab59945)参考文献

This product has been referenced in:
  • Sinclair DC  et al. Leiomyoma Simultaneously Impair Endometrial BMP-2-Mediated Decidualization and Anticoagulant Expression through Secretion of TGF-{beta}3. J Clin Endocrinol Metab 96:412-21 (2011). IHC-P ; Human . Read more (PubMed: 21084396) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cultured Human Lymphoblasts)
Loading amount 40 µg
Specification Cultured Human Lymphoblasts
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"