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Synthetic peptide corresponding to Human BMPR1A aa 166-195 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab38560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25093411|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
Immunohistochemical (formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling BMPR1A with ab38560. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
ICC/IF image of ab38560 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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