Anti-BMPER抗体(ab73900)
Key features and details
- Rabbit polyclonal to BMPER
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-BMPER抗体 -
描述
兔多克隆抗体to BMPER -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse BMPER aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab74993) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73900于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 75,110 kDa (predicted molecular weight: 76 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75,110 kDa (predicted molecular weight: 76 kDa). |
靶标
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功能
Inhibitor of bone morphogenetic protein (BMP) function, it may regulate BMP responsiveness of osteoblasts and chondrocytes. -
组织特异性
Highly expressed in lung, and brain and also in primary chondrocytes. -
疾病相关
Defects in BMPER are the cause of diaphanospondylodysostosis (DSD) [MIM:608022]. A rare, recessively inherited, perinatal lethal skeletal disorder. The primary skeletal characteristics of the phenotype include a small chest, abnormal vertebral segmentation, and posterior rib gaps containing incompletely differentiated mesenchymal tissue. Consistent craniofacial features include ocular hypertelorism, epicanthal folds, a depressed nasal bridge with a short nose, and low-set ears. The most commonly described extraskeletal finding is nephroblastomatosis with cystic kidneys, but other visceral findings have been described in some cases. -
序列相似性
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 5 VWFC domains.
Contains 1 VWFD domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 168667 Human
- Entrez Gene: 73230 Mouse
- Entrez Gene: 300455 Rat
- Omim: 608699 Human
- SwissProt: Q8N8U9 Human
- SwissProt: Q8CJ69 Mouse
- Unigene: 660998 Human
- Unigene: 735847 Human
see all -
别名
- BMP binding endothelial regulator antibody
- BMP binding endothelial regulator precursor protein antibody
- BMP-binding endothelial regulator protein antibody
see all
图片
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ab73900 staining BMPER in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab73900 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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All lanes : Anti-BMPER antibody (ab73900) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 110,75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
BMPER has a predicted molecular weight of 76 kDa, however it is extensively glycosylated and has been seen to run in the 100 kDa region (other commercially available antibodies).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab73900 尚未被引用在任何文献中。