概述

  • 产品名称
  • 描述
    兔多克隆抗体to BMP8a
  • 经测试应用
    适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human BMP8a.

  • 阳性对照
    • This antibody gave a positive signal in human epididymus formalin fixed paraffin embedded tissue section within Immunohistochemistry.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • 序列相似性
    Belongs to the TGF-beta family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BMP-8A antibody
    • BMP8 antibody
    • Bmp8a antibody
    • BMP8A_HUMAN antibody
    • Bone morphogenetic protein 8A antibody
    • FLJ14351 antibody
    • FLJ45264 antibody
    • OP 2 antibody
    • Osteogenic protein 2 antibody
    see all

图片

  • IHC image of ab60290 staining in human epididymus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab60290, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Testes)
Specification
Testes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 03 2012

Application
Western blot
Sample
Rat Tissue lysate - whole (Adult Testes, brain)
Loading amount
20 µg
Specification
Adult Testes, brain
Gel Running Conditions
Reduced Denaturing (10% Poly acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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