Anti-BMP7抗体(ab84684)
Key features and details
- Rabbit polyclonal to BMP7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BMP7抗体
参阅全部 BMP7 一抗 -
描述
兔多克隆抗体to BMP7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Horse, Chicken, Pig -
免疫原
Synthetic peptide corresponding to Human BMP7 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104212) -
阳性对照
- Recombinant Human BMP7 protein (ab87055) can be used as a positive control in WB. This antibody gave a positive signal in both Human and Rat Brain Tissue Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84684于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. -
组织特异性
Expressed in the kidney and bladder. Lower levels seen in the brain. -
序列相似性
Belongs to the TGF-beta family. -
发展阶段
Expressed in the developing eye, brain and ear during embryogenesis. -
翻译后修饰
Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014). -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 395494 Chicken
- Entrez Gene: 655 Human
- Entrez Gene: 12162 Mouse
- Entrez Gene: 492315 Pig
- Entrez Gene: 85272 Rat
- Omim: 112267 Human
- SwissProt: P18075 Human
- SwissProt: P23359 Mouse
see all -
别名
- BMP 7 antibody
- BMP-7 antibody
- Bmp7 antibody
see all
图片
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All lanes : Anti-BMP7 antibody (ab84684) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 10 kDa, 33 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
IHC image of BMP7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84684, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Anti-BMP7 antibody (ab84684) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab84684 被引用在 5 文献中.
- Niikura T et al. Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with the Masquelet technique. J Orthop Surg Res 17:29 (2022). PubMed: 35033126
- Kim S et al. Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis. J Cell Mol Med 24:13507-13522 (2020). PubMed: 33079436
- Kim S et al. Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta-Mediated Epithelial-Mesenchymal Transition. Front Pharmacol 11:591275 (2020). PubMed: 33364962
- Li C et al. Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug. Biomaterials 226:119541 (2020). PubMed: 31634652
- Sun N et al. Monocrotaline pyrrole enhanced bone morphogenetic protein 7 signaling transduced by alternative activin A receptor type 2A in pulmonary arterial smooth muscle cells. Eur J Pharmacol 863:172679 (2019). PubMed: 31542483