概述

  • 产品名称Anti-BMP7抗体
    参阅全部 BMP7 一抗
  • 描述
    兔多克隆抗体to BMP7
  • 特异性This antibody reacts with BMP7.
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.

  • 阳性对照

应用

Our Abpromise guarantee covers the use of ab56023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • 组织特异性Expressed in the kidney and bladder. Lower levels seen in the brain.
  • 序列相似性Belongs to the TGF-beta family.
  • 发展阶段Expressed in the developing eye, brain and ear during embryogenesis.
  • 翻译后修饰Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all

Anti-BMP7 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
    Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
    Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml

    Lane 1 : Cell lysate prepared from BMP7 transfected 293T cells
    Lane 2 : Cell lysate prepared from BMP7 transfected 293T cells
    Lane 3 : Cell lysate prepared from BMP7 transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 49 kDa
  • ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution

    Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
    Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
    Lane 3 : Rat Brain Whole Tissue (age P21) Lysate
    Lane 4 : Rat Brain Whole Tissue (age P21) Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 30 seconds

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa

    See Abreview

  • ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

    See Abreview

Anti-BMP7 antibody (ab56023)参考文献

This product has been referenced in:
  • Sharma S  et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone. J Biol Chem 291:19351-63 (2016). Read more (PubMed: 27422817) »
  • Zhao XK  et al. Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats. World J Gastroenterol 20:14875-83 (2014). Rat . Read more (PubMed: 25356047) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Sample Rat Tissue lysate - whole (Rat liver)
Specification Rat liver
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 10 2014

Thank you for your enquiry.

All tested applications and species covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Therefore, I can c...

Read More

Thank you for contacting us. I have checked the image and can say that there are many unnecessary bands on the gel. I am happy to help you however I would need more information about the used protocol. Please answer my following questions;

W...

Read More

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Brain Lysate)
Loading amount 20 µg
Specification Brain Lysate
Gel Running Conditions Reduced Non-Denaturing (Native) (15% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Mar 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (e18 Skeleton)
Specification e18 Skeleton
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"