概述

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab27569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 49 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to BMP7 (Biotin) (ab84030). Use as Capture antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
Neutralising Use at an assay dependent dilution.

靶标

  • 功能Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • 组织特异性Expressed in the kidney and bladder. Lower levels seen in the brain.
  • 序列相似性Belongs to the TGF-beta family.
  • 发展阶段Expressed in the developing eye, brain and ear during embryogenesis.
  • 翻译后修饰Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all

Anti-BMP7 antibody 图像

  • ICC/IF image of ab27569 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of Rat primary hepatocytes culture cells stained with ab27569. The cells were fixed with 4% PFA and incubated in  5% normal donkey serum in 0.1% PBS- and triton X100  for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMP7 antibody (ab27569)参考文献

This product has been referenced in:
  • Heinonen AM  et al. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7. BMC Neurosci 15:38 (2014). WB ; Mouse . Read more (PubMed: 24618040) »
  • Roxburgh SA  et al. Allelic depletion of grem1 attenuates diabetic kidney disease. Diabetes 58:1641-50 (2009). WB ; Human, Mouse . Read more (PubMed: 19401426) »

See all 3 Publications for this product

Product Wall

Thank you for your response.

You may wish to look at http://www.biocompare.com website to see what alternative products are on the market.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your enquiry and your interest in our products.

I am very sorry to inform you that as the disclaimer states on the bottom of each of our datasheets: "all our products are for research use only and are not intended for diagn...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary hepatocytes culture)
Specification Primary hepatocytes culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Tritox100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (OVCAR5 cell)
Loading amount 50 µg
Specification OVCAR5 cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

提交于 Jun 29 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"