概述

性能

应用

Our Abpromise guarantee covers the use of ab93852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

靶标

图片

  • All lanes : Anti-BMP5 antibody (ab93852) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human bone tumor tissue lysate - total protein (ab29359)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 50 kDa (possible cleavage fragment)


    Exposure time: 15 minutes


    The band observed at 50 kDa could potentially be a cleaved form of BMP5 due to the presence of a 30 amino acid signal peptide.

实验方案

文献

ab93852 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab93852.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册