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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4.
(Peptide available as ab40140.)
Our Abpromise guarantee covers the use of ab39973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24376809|
|ICC/IF||Use a concentration of 1 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat bladder tissue sections labeling BMP4 with ab39973 at 1/100 dilution. The tissue was fixed in 10% buffered formalin overnight, processed and embedded in paraffin. Serial 6 µm sagittal tissue sections were sliced along the cranial-caudal axis of the bladder. (u) urothelium; (lp) lamina propria.
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