概述

性能

应用

Our Abpromise guarantee covers the use of ab39973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 24376809
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • 组织特异性Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • 疾病相关Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • zgc:100779 antibody
    • BMP 2B antibody
    • BMP 4 antibody
    • BMP-2B antibody
    • BMP-4 antibody
    • BMP2B antibody
    • BMP2B1 antibody
    • BMP4 antibody
    • BMP4_HUMAN antibody
    • Bone morphogenetic protein 2B antibody
    • Bone morphogenetic protein 4 antibody
    • DVR4 antibody
    • MCOPS6 antibody
    • MGC100779 antibody
    • OFC11 antibody
    • zbmp-4 antibody
    • ZYME antibody
    see all

Anti-BMP4 antibody 图像

  • All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.


  • Performed under reducing conditions.

    Predicted band size : 47 kDa
    ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution

    Lane 1 : mouse brain
    Lane 2 : mouse brain
    Lane 3 : mouse brain
    Lane 4 : mouse brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa


    Exposure time : 1 minute

    This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

  • ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMP4 antibody (ab39973)参考文献

This product has been referenced in:
  • Chow A  et al. CD169? macrophages provide a niche promoting erythropoiesis under homeostasis and stress. Nat Med 19:429-36 (2013). Read more (PubMed: 23502962) »
  • Sun L  et al. Defining the role of fluid shear stress in the expression of early signaling markers for calcific aortic valve disease. PLoS One 8:e84433 (2013). WB, IHC-Fr ; Human . Read more (PubMed: 24376809) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 28 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Xenopus laevis Tissue sections (NF stage embryos)
Antigen retrieval step Heat mediated
Permeabilization Yes - PBT (0.1% Triton in PBS)
Specification NF stage embryos
Blocking step (agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 07 2015

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Cell lysate - whole cell (Gastrocnemius)
Specification Gastrocnemius
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Apr 11 2014

Thank you for contacting Abcam.


I have requested that a vial of ab124715 be shipped out immediately in Cedarlane's next order. I will confirm this shortly.


I apologize for any inconvenience this has caused. Please do not...

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Thank you for contacting us.

As we discussed on the phone, you may be able to improve your WB results by switching from milk to BSA and by using a higher concentration of primary antibody (1:500 for example). If you are unable to improve your...

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I am very pleased to hear you would like to accept our offer and test ab39973 inIHC-Fr on pig. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, p...

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Thank you very much for your interest in ab39973. I can confirm that this antibody has not yet been tested with sheep samples The immunogen shows 84% homology with the sequence you are interested in. We usually expect a cross-reaction for an antibody f...

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Thank you for your enquiry. The ab92855 BMP4 protein (MBP) and ab39973 BMP4 antibody have both been tested and guaranteed in western blot. The two products have not been specifically tested together in the laboratory, but as the protein is full len...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 28 2011

Thank you for contacting us. The gallus BMP4 sequence shares 11 of 13 amino acids with the immunogen for ab39973: + ++++ ++++++ That may be sufficient for cross-reaction but the only species that have been tested, to our knowledge, are human mouse and ...

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