Anti-BMP4抗体(ab155033)
Key features and details
- Rabbit polyclonal to BMP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-BMP4抗体
参阅全部 BMP4 一抗 -
描述
兔多克隆抗体to BMP4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 1-287 of Human BMP4 (UniProt ID: P12644).
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阳性对照
- Recombinant Human BMP4 protein (ab92855) can be used as a positive control in WB. A549, NIH3T3 and JC whole cell lysates; HeLa cells; Human MDA-MB468 xenograft tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. -
组织特异性
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. -
疾病相关
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 652 Human
- Entrez Gene: 12159 Mouse
- Omim: 112262 Human
- SwissProt: P12644 Human
- SwissProt: P21275 Mouse
- Unigene: 68879 Human
- Unigene: 6813 Mouse
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别名
- zgc:100779 antibody
- BMP 2B antibody
- BMP 4 antibody
see all
图片
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Anti-BMP4 antibody (ab155033) at 1/1000 dilution + A549 whole cell lysates at 30 µg
Predicted band size: 47 kDa
10% SDS-PAGE -
All lanes : Anti-BMP4 antibody (ab155033) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysates
Lane 2 : JC whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS-PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells, labeling BMP4 using ab155033 at 1/100 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human MDA-MB468 xenograft tissue, labeling BMP4 using ab155033 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab155033 被引用在 4 文献中.
- Walker H et al. Conditional Deletion of AP-2β in the Periocular Mesenchyme of Mice Alters Corneal Epithelial Cell Fate and Stratification. Int J Mol Sci 22:N/A (2021). PubMed: 34445433
- Cao W et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). PubMed: 29651293
- Wang G et al. Osteopontin, Bone Morphogenetic Protein-4, and Vitamin D Receptor Gene Polymorphisms in the Susceptibility and Clinical Severity of Spinal Tuberculosis. Cell Physiol Biochem 41:1881-1893 (2017). PubMed: 28376475
- Gao Y et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014). ICC ; Mouse . PubMed: 24803588