为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Recombinant full length protein expressed in E. coli (Human).
Our Abpromise guarantee covers the use of ab6285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825|
|ICC/IF||Use a concentration of 1 µg/ml.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rabbit periosteum labeling BMP2 with ab6285 at 1/100 dilution. The tissue was fixed with paraformaldehyde, followed by blocking in 5% serum for 20 minutes. The tissue was incubated with ab6285 for 12 hours at 4°C in antibody dilution solution. An undiluted polyclonal goat-anti mouse HRP conjugate secondary antibody was used.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"