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Recombinant full length protein expressed in E. coli (Human).
Our Abpromise guarantee covers the use of ab6285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825|
|ICC/IF||Use a concentration of 1 µg/ml.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"