Anti-BMP2抗体[65529.111] (ab6285)


  • 产品名称Anti-BMP2抗体[65529.111]
    参阅全部 BMP2 一抗
  • 描述
    小鼠单克隆抗体[65529.111] to BMP2
  • 特异性Monoclonal anti-BMP-2 recognizes recombinant human BMP-2 by ELISA and immunoblotting. The antibody shows approximately 25% cross-reactivity with recombinant human BMP-4. Mouse and rat species reactivity comes from publications. Please note that Abcam does not batch test in these species.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, Indirect ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant full length protein expressed in E. coli (Human).

  • 阳性对照
  • 常规说明This product is supplied as 100ug/vial. The concentration range may vary from 0.5mg/ml to 1mg/ml.



Our Abpromise guarantee covers the use of ab6285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825
ICC/IF Use a concentration of 1 µg/ml.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.


Anti-BMP2 antibody [65529.111] 图像

  • ab66285 staining BMP2 in Human small intestine tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes. Antigen retrieval was by heat mediation in citrate (pH6.0). Samples were incubated with primary antibody (1/100 in diluent buffer) for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody

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  • ICC/IF image of ab6285 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMP2 antibody [65529.111] (ab6285)参考文献

This product has been referenced in:
  • Ravindran S  et al. Biomimetically enhanced demineralized bone matrix for bone regenerative applications. Front Physiol 6:292 (2015). IHC ; Mouse . Read more (PubMed: 26557093) »
  • Chapellier M  et al. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response. Stem Cell Reports 4:239-54 (2015). IHC-P ; Human . Read more (PubMed: 25601208) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5%
Antigen retrieval step None
Sample Rabbit Tissue sections (Periosteum of rabbit)
Specification Periosteum of rabbit
Permeabilization No
Fixative Paraformaldehyde

Mr. 盛世中方 Zhu

Verified customer

提交于 Jan 07 2014

Thank you for your enquiry.

We do not have set expiration dates for our products.

Most un-conjugated antibodies are stable and can last for several years if stored properly, so we strongly recommend that you follow the storage inst...

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Thank you for contacting Abcam

The immunogen for ab6285 was recombinant full length protein expressed in E. coli (Human), most likely the pro-BMP2 (= mature BMP2 plus propeptide region attached) form. The exact epitope sequence has not been de...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas après quelques mois stocké à -20ºC.
Comme convenu j'ai mis en place...

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Thank you for contacting Abcam.

I have had the opportunity to speak with the lab to try and clarify the statement,"The antibody recognizes recombinant human BMP-2. It shows approximately 25% cross-reactivity with recombinant human BMP-4...

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Merci de nous avoir contactés.

J'ai retrouvé le document que j'avais créé fin d'année 2011 concernant les explications en français pour le stockage des anticorps. Voir pièce jointe.


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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryo)
Specification embryo
Fixative Formaldehyde
Permeabilization Yes - 3% triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 10 2012

Thank you for your inquiry and I apologize that we were not able to receive your earlier phone call.

I have attached the dtatsheets you have requested.

This is where you can find our mouse on mouse staining protocol. Read More

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to cusotmers. ...

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I am very pleased to hear you would like to accept our offer and test both antibodies in chicken. The discount codes that I'm using are listed above. Each code will be worth the price you pay for each antibody and can be used with any single future P...

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