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Our Abpromise guarantee covers the use of ab56645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
ab56645 staining BMP11 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% BSA + 0.2% Triton X-100 in PBS and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (diluted in 1.5% Goat serum + 1% BSA + 0.2% Triton X-100 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Primary antibody dilution:
Top: left - 1/100, middle - 1/200, right - 1/300.
Bottom: left - 1/1000, middle - 1/3000, right - negative control.
ab56645 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"