概述

  • 产品名称
  • 描述
    兔多克隆抗体to BMP1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide based on the carboxyterminal end of the mTld form of Human BMP1. (Peptide available as ab41291.)

性能

应用

Our Abpromise guarantee covers the use of ab38953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 111 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

靶标

  • 相关性
    Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • BMP 1 antibody
    • Bone morphogenetic protein 1 antibody
    • Mammalian tolloid protein antibody
    • mTld antibody
    • OI13 antibody
    • PCOLC antibody
    • PCP antibody
    • PCP2 antibody
    • Procollagen C endopeptidase antibody
    • Procollagen C proteinase antibody
    • TLD antibody
    • Tolloid, Drosophila, homolog of antibody
    see all

文献

This product has been referenced in:
  • Voorhees AP  et al. Building a better infarct: Modulation of collagen cross-linking to increase infarct stiffness and reduce left ventricular dilation post-myocardial infarction. J Mol Cell Cardiol 85:229-39 (2015). Read more (PubMed: 26080361) »
  • Zamilpa R  et al. CC chemokine receptor 5 deletion impairs macrophage activation and induces adverse remodeling following myocardial infarction. Am J Physiol Heart Circ Physiol 300:H1418-26 (2011). WB ; Mouse . Read more (PubMed: 21297029) »

See all 3 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.2% Triron X100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Sep 27 2017

Thank you for your email. The Abreview discount code is now valid as you the Abreview has been received and published. Please submit your order with the discount code for it to be applied to your next order. Thank you for your feedback ...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney)
Loading amount
30 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Sebastian Brings

Verified customer

提交于 Oct 21 2011

Thank you very much for your interest in ab38953. To our knowledge, ab38953 has not been tested in rat. Unfortunately I can not provide a sequence alignment neither, as the immunogen sequence is proprietary information of the laboratory producing t...

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Thank you for your enquiry. The lysates used to test this antibody for Western blotting were from RD cells and A2058 cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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