概述

  • 产品名称
  • 描述
    兔多克隆抗体to BMP1
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Dog, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    RYTSTKFQDTLHSRK

    conjugated to KLH, corresponding to C terminal amino acids 972-986 of Human BMP1.

  • 阳性对照
    • Human Skin and Colon tissues.

应用

Our Abpromise guarantee covers the use of ab118520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1 - 1/1000.

靶标

  • 相关性
    Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • BMP 1 antibody
    • Bone morphogenetic protein 1 antibody
    • Mammalian tolloid protein antibody
    • mTld antibody
    • OI13 antibody
    • PCOLC antibody
    • PCP antibody
    • PCP2 antibody
    • Procollagen C endopeptidase antibody
    • Procollagen C proteinase antibody
    • TLD antibody
    • Tolloid, Drosophila, homolog of antibody
    see all

图片

  • Immunohistochemical analysis of BMP1 expression in Formalin-Fixed, Paraffin-Embedded Human Skin using 10 µg/ml ab118520 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of BMP1 expression in Formalin-Fixed, Paraffin-Embedded Human Colon using 10 µg/ml ab118520 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

This product has been referenced in:
  • Noda S  et al. Transforming growth factor-ß signaling is upregulated in sporadic inclusion body myositis. Muscle Nerve N/A:N/A (2016). Read more (PubMed: 27623743) »
  • Olderøy MØ  et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . Read more (PubMed: 24626259) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human aorta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate 10mM
Permeabilization
Yes - Triton X 0.5% for 15min at room temperature
Specification
Human aorta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0)
Sample
Human Tissue sections (Human Ovary)
Specification
Human Ovary
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

提交于 Mar 17 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Microwave 5min, Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
Sample
Mouse Tissue sections (mouse tail (chondrocytes))
Specification
mouse tail (chondrocytes)
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Jan 23 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Microwave Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0)
Sample
Human Tissue sections (human cartilage)
Specification
human cartilage
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Dec 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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