概述

  • 产品名称
  • 描述
    兔多克隆抗体to Bmi1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

  • 阳性对照
    • K562 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20083111
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).
IHC-Fr 1/300.
IP 1/2000.

靶标

  • 功能
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 序列相似性
    Contains 1 RING-type zinc finger.
  • 翻译后修饰
    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    see all

Anti-Bmi1 antibody 图像

  • ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
    Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
    Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : K562 cell lysate


    Predicted band size : 37 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

Anti-Bmi1 antibody (ab38295)参考文献

This product has been referenced in:

See all 14 Publications for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Rat Cell lysate - whole cell (Oligodendrocytes)
Specification
Oligodendrocytes
Total protein in input
30 µg
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Abcam user community

Verified customer

提交于 Dec 09 2013

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12.5%)
Sample
Rat Cell lysate - whole cell (Oligodendrocyte)
Specification
Oligodendrocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 09 2013

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample
Rat Cell (Fibroblast)
Specification
Fibroblast
Permeabilization
Yes - 0.01% triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 30 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MKN45)
Loading amount
30 µg
Specification
MKN45
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 14 2013

Thank you for your response.
I have discussed this enquiry with my colleagues.
A BLAST with the immunogen in the human proteome does not predict cross-reactivity with any other proteins, just BMI-1 and partial BMI-1 sequences. It could be a c...

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Thank you for your enquiry regarding ab38295 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
My colleague has asked me to look after her customers ...

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Vielen Dank für Ihre Anfrage.
Es tut mir Leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Laut unseren Unterlagen haben wir den Antikörper nicht selber in der IHC getestet, sondern haben diese Applikation aufgrund einer Publikation au...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

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Thank you very much for following this up with the customer. I am sorry that the change in the blocking buffer did not improve the results.

I would still like to recommend to the customer to reduce the samples for the Western blot either with...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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