Anti-Bmi1抗体(ab115251)
Key features and details
- Goat polyclonal to Bmi1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Bmi1抗体
参阅全部 Bmi1 一抗 -
描述
山羊多克隆抗体to Bmi1 -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
免疫原
Synthetic peptide:
DSGSDKANSPAGG
, corresponding to amino acids 252-264 of Human Bmi1 -
阳性对照
- Recombinant Human Bmi1 protein (ab114169) can be used as a positive control in WB. Human Skeletal Muscle tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115251于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 554342 Cat
- Entrez Gene: 493647 Chicken
- Entrez Gene: 510666 Cow
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 100173052 Orangutan
- Entrez Gene: 307151 Rat
see all -
别名
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
图片
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Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ab115251 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115251 staining Bmi1 in Mouse Pancreatic intraepithelial neoplasia tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% BSA for 60 minutes at 26°C and antigen retrieval was by heat mediation in Tris-based buffer. The sample was incubated with primary antibody (1/200 in 10% BSA in TBST) at 4°C for 16 hours. A HRP-conjugated Donkey anti-goatpolyclonal (1/1000) was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab115251 被引用在 3 文献中.
- Tanimura Y et al. Long-term model of colitis-associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells. Oncol Lett 21:7 (2021). PubMed: 33240413
- Yoshioka T et al. Bmi1 marks gastric stem cells located in the isthmus in mice. J Pathol 248:179-190 (2019). PubMed: 30689202
- Qiu M et al. MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells. Exp Ther Med 13:1329-1336 (2017). WB ; Human . PubMed: 28413473