Anti-BMAL1抗体- ChIP Grade (ab3350)


  • 产品名称Anti-BMAL1抗体- ChIP Grade
    参阅全部 BMAL1 一抗
  • 描述
    兔多克隆抗体to BMAL1 - ChIP Grade
  • 特异性Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster tissues as well as recombinant human BMAL 1.
  • 经测试应用适用于: ICC/IF, WB, IP, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human
    预测可用于: Horse, Chicken
  • 免疫原

    Synthetic peptide corresponding to Mouse BMAL1 aa 582-594.


    (Peptide available as ab4959)


Our Abpromise guarantee covers the use of ab3350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
EMSA Use at an assay dependent concentration. PubMed: 22210883
WB 1/200. Predicted molecular weight: 69.4 kDa.Can be blocked with BMAL1 peptide (ab4959). This antibody detects a protein which corresponds to the product of a hamster BMAL 1 fusion construct overexpressed in E. coli (110 kDa band)as well as recombinant human BMAL1.
IP Use at an assay dependent concentration. PubMed: 23547261
ChIP Use at an assay dependent concentration. PubMed: 18039858


  • 功能Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK
    ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. Plays a role in normal pancreatic beta-cell function; regulates glucose-stimulated insulin secretion via the regulation of antioxidant genes NFE2L2/NRF2 and its targets SESN2, PRDX3, CCLC and CCLM. Negatively regulates the mTORC1 signaling pathway; regulates the expression of MTOR and DEPTOR. Controls diurnal oscillations of Ly6C inflammatory monocytes; rhythmic recruitment of the PRC2 complex imparts diurnal variation to chemokine expression that is necessary to sustain Ly6C monocyte rhythms. Regulates the expression of HSD3B2, STAR, PTGS2, CYP11A1, CYP19A1 and LHCGR in the ovary and also the genes involved in hair growth. Plays an important role in adult hippocampal neurogenesis by regulating the timely entry of neural stem/progenitor cells (NSPCs) into the cell cycle and the number of cell divisions that take place prior to cell-cycle exit. Regulates the circadian expression of CIART and KLF11. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina.
  • 组织特异性Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
  • 序列相似性Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
    O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.
    Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.
    Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry.
    Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • 细胞定位Nucleus. Cytoplasm. Nucleus, PML body. Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARNT like protein 1 brain and muscle antibody
    • Arntl antibody
    • Aryl hydrocarbon receptor nuclear translocator like antibody
    • Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
    • Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
    • Basic helix loop helix PAS orphan MOP3 antibody
    • Basic helix loop helix PAS protein MOP3 antibody
    • Basic-helix-loop-helix-PAS protein MOP3 antibody
    • bHLH PAS protein JAP3 antibody
    • bHLH-PAS protein JAP3 antibody
    • bHLHe5 antibody
    • BMAL 1 antibody
    • BMAL1_HUMAN antibody
    • BMAL1c antibody
    • Brain and muscle ARNT like 1 antibody
    • Brain and muscle ARNT-like 1 antibody
    • CG8727 PA antibody
    • Class E basic helix-loop-helix protein 5 antibody
    • cycle antibody
    • JAP 3 antibody
    • JAP3 antibody
    • Member of PAS protein 3 antibody
    • Member of PAS superfamily 3 antibody
    • MGC47515 antibody
    • MOP 3 antibody
    • MOP3 antibody
    • PAS domain-containing protein 3 antibody
    • PASD 3 antibody
    • PASD3 antibody
    • TIC antibody
    see all

Anti-BMAL1 antibody - ChIP Grade 图像

  • All lanes : Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1/500 dilution

    Lane 1 : U251 cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 69.4 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
  • Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 69.4 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.
  • ICC/IF image of ab3350 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMAL1 antibody - ChIP Grade (ab3350)参考文献

This product has been referenced in:
  • Wang Q  et al. Changes in the daily rhythm of lipid metabolism in the diabetic retina. PLoS One 9:e95028 (2014). IHC-P ; Rat . Read more (PubMed: 24736612) »
  • Kyoko OO  et al. Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitis. PLoS One 9:e98016 (2014). WB, ChIP ; Mouse . Read more (PubMed: 24845399) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hepatocytes)
Permeabilization Yes - : 0.1% Triton X-100 in TBS for 5-10 minutes
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde

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Verified customer

提交于 Dec 11 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cell (Hepatocyte)
Specification Hepatocyte
Permeabilization No
Fixative Paraformaldehyde

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Verified customer

提交于 Mar 20 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification primary hepatoctyes
Blocking step I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

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提交于 Jul 05 2013

Thank you for contacting us.

I could suggest buying ab150035.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with an...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

This antibody has been tested with Hela cells and is guaranteed for working in WB including other cell line lysates. We can certainly pro...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunofluorescence
Sample Mouse Tissue sections (Brain Suprachiasmatic nuclei)
Specification Brain Suprachiasmatic nuclei
Fixative Paraformaldehyde
Antigen retrieval step None
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

Abcam user community

Verified customer

提交于 Oct 10 2005

Thank you for your enquiry. We currently do not have an image of the western blot with ab3350, all the information, including images, we have for all our products is listed on the on-line datasheets for your convenience and we update these as soon as w...

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