Anti-Blood Group Lewis b抗体[2-25LE] (ab3968)

概述

  • 产品名称
    Anti-Blood Group Lewis b抗体[2-25LE]
    参阅全部 Blood Group Lewis b 一抗
  • 描述
    小鼠单克隆抗体[2-25LE] to Blood Group Lewis b
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 纯化说明
    Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    2-25LE
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200 - 1/400. PubMed: 17202152
Flow Cyt Use at an assay dependent concentration. PubMed: 23144050
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
  • 细胞定位
    Plasma membrane - adsorbed onto the surface of erythrocytes.
  • 数据库链接
  • 别名
    • gastric mucin antibody
    • leB antibody
    • lewis antigen system antibody
    • lewis b antibody
    • lewis B blood group antigen antibody
    • lewisb antibody
    • major airway glycoprotein antibody
    • MUC5 antibody
    • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
    • mucin 5AC, oligomeric mucus/gel-forming antibody
    • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
    • mucin-5 subtype AC, tracheobronchial antibody
    • TBM antibody
    • tracheobronchial mucin antibody
    see all

Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)参考文献

This product has been referenced in:
  • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . Read more (PubMed: 24998328) »
  • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). Read more (PubMed: 25048705) »

See all 13 Publications for this product

Product Wall

Thank you for contacting us.

We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human colorectal cell line C170)
Specification
Human colorectal cell line C170
Fixative
Formaldehyde
Permeabilization
No
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提交于 Feb 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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