Anti-Blood Group Lewis a抗体[7LE] (ab3967)

概述

  • 产品名称Anti-Blood Group Lewis a抗体[7LE]
    参阅全部 Blood Group Lewis a 一抗
  • 描述
    小鼠单克隆抗体[7LE] to Blood Group Lewis a
  • 经测试应用适用于: Flow Cyt, ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Tissue/ cell preparation (Human). Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号7LE
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/200 - 1/400. PubMed: 17202152

靶标

  • 相关性Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • 细胞定位Cytoplasmic
  • 别名
    • Lewis A antigen antibody

Anti-Blood Group Lewis a antibody [7LE] 图像

  • ab3967 staining Blood Group Lewis a in the Human colorectal cell line C170 by Flow Cytometry. Cells were prepared by trypsinisation in PBS prior to incubation with the primary antibody (1 µg/ml in PBS) for 1 hour at 4°C. A FITC-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Ungated

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  • ab3967 staining Blood Group Lewis a in Human colorectal carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were PFA-fixed and subjected to heat-mediated antigen retrieval in citric acid (pH 6.0, citrate buffer) prior to blocking with 2% serum for 20 minutes at 22°C. The primary antibody was used undiluted and incubated with the sample for 1 hour at 22°C. An biotinylated universal ABC kit was used for detection.

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Anti-Blood Group Lewis a antibody [7LE] (ab3967)参考文献

This product has been referenced in:
  • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . Read more (PubMed: 24998328) »
  • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). Read more (PubMed: 25048705) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal carcinoma)
Specification Colorectal carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
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Verified customer

提交于 Mar 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Human colorectal cell line C170)
Specification Human colorectal cell line C170
Preparation Cell harvesting/tissue preparation method: Typsinisation
Sample buffer: PBS
Permeabilization No
Gating Strategy Ungated
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Abcam user community

Verified customer

提交于 Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Intestine)
Loading amount 50 µg
Specification Intestine
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Verified customer

提交于 Feb 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"