Anti-Blood Group AB antigen抗体[Z5H-2/Z2A] (ab24223)

概述

  • 产品名称Anti-Blood Group AB antigen抗体[Z5H-2/Z2A]
  • 描述
    小鼠单克隆抗体[Z5H-2/Z2A] to Blood Group AB antigen
  • 经测试应用适用于: ELISA, WB, Flow Cyt, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Antigen A B of blood cells.

性能

应用

Our Abpromise guarantee covers the use of ab24223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
Flow Cyt
IP
ICC/IF
  • 应用说明ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (PMID 19608744).
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Anti-Blood Group AB antigen antibody [Z5H-2/Z2A] 图像

    • ab24223 staining Blood Group AB antigen in A549 cells by Immunocytochemistry/ Immunofluorescence.
      Cells were rinsed in PBS and fixed in 4% paraformaldehyde in PBS (15 minutes), rinsed, and blocked in 5% lamb serum in PBS/Tween 20 (15 minutes, room temperature). Cells were rinsed three times in PBS before application of primary antibodies. ab24223 diluted 1/400 in 5% lamb serum in PBS and applied to the fixed cells overnight (4 °C). Specimens were rinsed three times in PBS, and Alexa 568-conjugated goat anti-mouse IgG secondary antibody diluted 1/800 in PBS was applied. Counterstained with DAPI.

    Anti-Blood Group AB antigen antibody [Z5H-2/Z2A] (ab24223)参考文献

    This product has been referenced in:
    • Higgins MA  et al. Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases. J Biol Chem 284:26161-73 (2009). ICC/IF ; Human . Read more (PubMed: 19608744) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab24223.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"