Anti-Blood Group A Antigen抗体[HE-195] (ab2522)

概述

  • 产品名称Anti-Blood Group A Antigen抗体[HE-195]
    参阅全部 Blood Group A Antigen 一抗
  • 描述
    小鼠单克隆抗体[HE-195] to Blood Group A Antigen
  • 特异性This antibody recognizes human blood group A antigens, including weak variants A3, AX, A3B, AXB
  • 经测试应用适用于: Agglutinationmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: Tissue Culture Supernatant
  • 纯度Tissue culture supernatant
  • 纯化说明Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • 克隆单克隆
  • 克隆编号HE-195
  • 骨髓瘤unknown
  • 同种型IgM
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Agglutination
  • 应用说明In agglutination tests, the minimum titre is 1:256 with erythrocytes of blood group A1
  • 靶标

    • 相关性Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
    • 细胞定位Cell Membrane
    • 数据库链接

    Anti-Blood Group A Antigen antibody [HE-195] (ab2522)参考文献

    This product has been referenced in:
    • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »

    See 1 Publication for this product

    Product Wall

    I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

    Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to con...

    Read More

    Hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"