Welcome to the Abcam blog

What you will learn

Our ICC webinar provides tips for:

• Cell culture and sample preparation
• ICC general staining procedure (Fixation and permeabilization, Antibody staining)
• Recommended controls
• Fluorescence
• Troubleshooting
• Antibody selection

Watch our immunocytochemistry webinar

Immunocytochemistry explained

Immunocytochemistry is a method for demonstrating the presence of protein within individual cells in cell culture. This is a useful application for many research areas which can be used to assess the specific localisation of proteins within the cell.

Western blot (WB), also called immunoblot, uses specific antibodies to analyze one protein in a sample containing multiple proteins. Proteins are separated from each other by size using gel electrophoresis, then transferred to a membrane and detected with a specific antibody. This technique is widely used in biological research, as it provides information about the molecular weight of a protein as well as relative differences in expression between samples.

Highlighted points in this webinar are:

• An introduction to chromatin and the ChIP technique
• ChIP Protocols
• Antibody selection
• Troubleshooting tips
• ChIP resources

ChIP is a very powerful technique that allows the localization of proteins on DNA regions in the genome. The principle is simple; the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a protein or protein modification are used to determine the relative abundance in the genome in vivo. The ChIP technique can be used in any area of research to further elucidate gene function and regulation in their native state.

Watch another of our popular webinars for free, 'Immunoprecipitation: Procedures, pitfalls and protocol tips'.

Immunoprecipitation is a method that enables the purification of a protein or protein complex. Proteins are physically isolated from a biological sample and subject to further analysis. IP can be used for a wide variety of applications such as protein-protein interactions, post-translational modifications, protein function, and expression profiling. Thus, this technique is a powerful tool for researchers studying cancer, development, cell signaling pathways, and disease. It also makes this an important tool to researchers studying biological process. Please view this webinar for a presentation on Immunoprecipitation procedures, pitfalls, and protocol tips.