Anti-BLNK (phospho Y84)抗体(ab79320)

概述

  • 产品名称Anti-BLNK (phospho Y84)抗体
    参阅全部 BLNK 一抗
  • 描述
    兔多克隆抗体to BLNK (phospho Y84)
  • 特异性ab79320 detects endogenous levels of BLNK only when phosphorylated at tyrosine 84.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 84 (EMYPVM).

  • 阳性对照
    • Human brain tissue and extracts from K562 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+)150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • 组织特异性Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 疾病相关Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • 序列相似性Contains 1 SH2 domain.
  • 翻译后修饰Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • 细胞定位Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGM4 antibody
    • B cell adapter containing SH2 domain protein antibody
    • B cell adapter containing Src homology 2 domain protein antibody
    • B cell adaptor containing SH2 domain antibody
    • B cell linker antibody
    • B cell linker protein antibody
    • B cell-specific adaptor protein antibody
    • B-cell activation antibody
    • B-cell adapter containing a SH2 domain protein antibody
    • B-cell adapter containing a Src homology 2 domain protein antibody
    • B-cell linker protein antibody
    • BASH antibody
    • Bca antibody
    • BLNK antibody
    • BLNK s antibody
    • BLNK_HUMAN antibody
    • Cytoplasmic adapter protein antibody
    • Ly 57 antibody
    • Ly57 antibody
    • Lymphocyte antigen 57 antibody
    • Lyw 57 antibody
    • Lyw57 antibody
    • MGC111051 antibody
    • SH2 domain-containing leukocyte protein, 65-KD antibody
    • SLP 65 antibody
    • SLP-65 antibody
    • SLP65 antibody
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
    • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
    • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
    see all

Anti-BLNK (phospho Y84) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody at 1/50 dilution in the presence and absence of immunizing phosphopeptide
  • All lanes : Anti-BLNK (phospho Y84) antibody (ab79320) at 1/500 dilution

    Lane 1 : Extracts from K562 cells at 30ug of total protein, treated with starved (24 hours)
    Lane 2 : Extracts from K562 cells at 30ug of total protein, treated with starved (24 hours) + 10ug of immunizing phosphopeptide


    Predicted band size : 51 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

Anti-BLNK (phospho Y84) antibody (ab79320)参考文献

ab79320 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The BLNK antibody ab79320 recognizes the sequence EMYPVM. This correlates to the phosphorylated tyrosine 84 in the UniProt sequence Q8WV28 and the NCBI sequenceNP_037446.1. Our system automatically sets the "Predicted ...

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