Anti-BLNK抗体[Y491] (ab32418)


  • 产品名称Anti-BLNK抗体[Y491]
    参阅全部 BLNK 一抗
  • 描述
    兔单克隆抗体[Y491] to BLNK
  • 特异性This antibody is specific for human BLNK.
  • 经测试应用适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human BLNK.

  • 阳性对照
    • Raji cell lysate and human spleen tissue slides.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500.
IHC-P Use at an assay dependent concentration.
IP 1/20.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
    • 组织特异性Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
    • 疾病相关Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
    • 序列相似性Contains 1 SH2 domain.
    • 翻译后修饰Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
    • 细胞定位Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AGM4 antibody
      • B cell adapter containing SH2 domain protein antibody
      • B cell adapter containing Src homology 2 domain protein antibody
      • B cell adaptor containing SH2 domain antibody
      • B cell linker antibody
      • B cell linker protein antibody
      • B cell-specific adaptor protein antibody
      • B-cell activation antibody
      • B-cell adapter containing a SH2 domain protein antibody
      • B-cell adapter containing a Src homology 2 domain protein antibody
      • B-cell linker protein antibody
      • BASH antibody
      • Bca antibody
      • BLNK antibody
      • BLNK s antibody
      • BLNK_HUMAN antibody
      • Cytoplasmic adapter protein antibody
      • Ly 57 antibody
      • Ly57 antibody
      • Lymphocyte antigen 57 antibody
      • Lyw 57 antibody
      • Lyw57 antibody
      • MGC111051 antibody
      • SH2 domain-containing leukocyte protein, 65-KD antibody
      • SLP 65 antibody
      • SLP-65 antibody
      • SLP65 antibody
      • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
      • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
      • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
      see all

    Anti-BLNK antibody [Y491] 图像

    • ab32418 at a 1:50 dilution staining BLNK in human spleen tissue using Immunohistochemistry, Paraffin Embedded Tissue.
    • Anti-BLNK antibody [Y491] (ab32418) at 1/500 dilution + Raji cell lysate.

      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-BLNK antibody [Y491] (ab32418)参考文献

    ab32418 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32418.
    Please use the links above to contact us or submit feedback about this product.