概述

  • 产品名称Anti-BLMH抗体
    参阅全部 BLMH 一抗
  • 描述
    小鼠单克隆抗体to BLMH
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: NMNKAERLTF GESLMTHAMT FTAVSEKDDQ DGAFTKWRVE NSWGEDHGHK GYLCMTDEWF SEYVYEVVVD RKHVPEEVLA VLEQEPIILP AWDPMGALA, corresponding to amino acids 356-454 of human BLMH (NP_000377) with a 26 kDa tag

  • 阳性对照
    • K562 cell lysate; recombinant tagged fragment of human BLMH (the immunogen).

性能

应用

Our Abpromise guarantee covers the use of ab77111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged BLMH is approximately 0.3ng/ml when used as a capture antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.

靶标

Anti-BLMH antibody 图像

  • Anti-BLMH antibody (ab77111) at 1/500 dilution + recombinant fragment of human BLMH (the immunogen) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 53 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-BLMH antibody (ab77111) at 1/500 dilution + K562 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

Anti-BLMH antibody (ab77111)参考文献

This product has been referenced in:
  • Baglo Y  et al. Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization. Biomed Res Int 2014:921296 (2014). WB . Read more (PubMed: 25101299) »

See 1 Publication for this product

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