Anti-BLK (phospho Y501)抗体(ab111889)

概述

  • 产品名称
    Anti-BLK (phospho Y501)抗体
    参阅全部 BLK 一抗
  • 描述
    兔多克隆抗体to BLK (phospho Y501)
  • 特异性
    ab111889 detects endogenous levels of BLK only when phosphorylated at tyrosine 501 (Human: Tyr501; Mouse: Tyr495; Rat: Tyr495).
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human BLK around the phosphorylation site of tyrosine 501 (R-Q-YP-E-L)

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab111889 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
  • 组织特异性
    Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
  • 疾病相关
    Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B lymphocyte kinase antibody
    • B lymphoid tyrosine kinase antibody
    • Blk antibody
    • BLK nonreceptor tyrosine kinase antibody
    • BLK_HUMAN antibody
    • EC 2.7.10.2 antibody
    • MGC 10442 antibody
    • MGC10442 antibody
    • MODY 11 antibody
    • MODY11 antibody
    • P55 BLK antibody
    • p55-BLK antibody
    • Tyrosine kinase B lymphocyte specific antibody
    • Tyrosine protein kinase antibody
    • Tyrosine-protein kinase BLK antibody
    see all

图片

  • ab111889 at 1/50 dilution staining BLK in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

文献

ab111889 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you very much for your interest in ab111889. To our knowledge, ab111889 has not been tested in WB. Please let me know if you have any questions about this offer and I would be happy to help you further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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