BLK overexpression 293T裂解液(全细胞) (ab94100)

概述

  • 产品名称BLK overexpression 293T裂解液(全细胞)
  • 描述
    BLK overexpression裂解液
  • 常规说明ab94100 is a 293T cell transfected lysate in which Human BLK has been transiently over-expressed using a pCMV-BLK plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Disease: Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Function: Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. Tissue specificity: Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.

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应用

Our Abpromise guarantee covers the use of ab94100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

BLK overexpression 293T lysate (whole cell) 图像

  • ab94100 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-BLK antibody (ab54866) at 1/500 dilution

    Lane 1 : BLK overexpression 293T lysate (whole cell) (ab94100)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

BLK overexpression 293T lysate (whole cell) (ab94100)参考文献

ab94100 has not yet been referenced specifically in any publications.

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