Anti-BLBP抗体- Neuronal Marker (ab32423)

概述

  • 产品名称Anti-BLBP抗体- Neuronal Marker
    参阅全部 BLBP 一抗
  • 描述
    兔多克隆抗体to BLBP - Neuronal Marker
  • 经测试应用适用于: IHC-FoFr, IHC-P, ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Zebrafish, Quail
    预测可用于: Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse BLBP.

    (Peptide available as ab32422.)

  • 阳性对照
    • Rat spinal cord from embryonic day 16; young zebrafish spinal cord This antibody gave a positive signal in the following mouse lysate: Brain (Mouse) Tissue Lysate - normal tissue, 0 days old

性能

应用

Our Abpromise guarantee covers the use of ab32423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/2000.
IHC-P 1/200 - 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Mouse BLBP peptide (ab32422).

Abcam recommends using milk as the blocking agent.

IHC-Fr Use at an assay dependent concentration. PubMed: 23675446

靶标

  • 功能B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
  • 组织特异性Expressed in brain and other neural tissues.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B FABP antibody
    • B-FABP antibody
    • BFABP antibody
    • BLBP antibody
    • Brain lipid binding protein antibody
    • Brain lipid-binding protein antibody
    • Brain-type fatty acid-binding protein antibody
    • DKFZp547J2313 antibody
    • FABP 7 antibody
    • FABP7 antibody
    • FABP7_HUMAN antibody
    • FABPB antibody
    • Fatty Acid Binding Protein 7 antibody
    • Fatty acid binding protein 7 brain antibody
    • Fatty acid binding protein brain antibody
    • Fatty acid-binding protein 7 antibody
    • Fatty acid-binding protein, brain antibody
    • Mammary derived growth inhibitor related antibody
    • Mammary-derived growth inhibitor related antibody
    • MRG antibody
    • OTTHUMP00000017119 antibody
    see all

Anti-BLBP antibody - Neuronal Marker 图像

  • BLBP detected on rat embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/200 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423 with low signal to noise ratio.BLBP detected on rat embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/200 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423 with low signal to noise ratio.
  • IHC-Fr image of Pax2 staining on zebrafish retina sections using ab32423 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab32423 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • Anti-BLBP antibody - Neuronal Marker (ab32423) at 1/250 dilution + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • ICC/IF image of ab32423 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32423, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • BLBP detected on mouse embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/300 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423.
  • Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for BLBP antibody - Neuronal Marker (ab32423)on Zebrafish Tissue sections (young adult whole body T/S). Antigen retrieval step: Heat mediated Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab32423 incubated at 1/500 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
  • Immunohistochemistical detection (on Formaldehyde /PFA-fixed paraffin-embedded sections) of BLBP protein using BLBP antibody - Neuronal Marker (ab32423) on Quail Tissue sections (developing spinal cord). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab32423 incubated at 1/500 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).

Anti-BLBP antibody - Neuronal Marker (ab32423)参考文献

This product has been referenced in:
  • Nagao M  et al. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun 7:11102 (2016). Mouse . Read more (PubMed: 27000654) »
  • Mills EA  et al. Astrocytes phagocytose focal dystrophies from shortening myelin segments in the optic nerve of Xenopus laevis at metamorphosis. Proc Natl Acad Sci U S A 112:10509-14 (2015). Read more (PubMed: 26240339) »

See all 18 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Muller glia cells)
Specification Retina, Muller glia cells
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

提交于 Aug 22 2014

Muchas gracias por enviarnos el protocolo relleno. Siento mucho que este anticuerpo os este dando problemas en IHC.

Para poder ayudaros a resolver el problema sufrido con el anticuerpo quisiera que me aclararais algunos detalles:

&i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary neural culture)
Specification Primary neural culture
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 21 2012

Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to investigate this particular case further for you, and also obtain more information for our quality reco...

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Thank you for your note.
First please do try and use the free of charge replacement and let me know how you are getting on with ab32423.
Keep in touch and good luck with the experiments!

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for your enquiry regarding abab32423 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise appl...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. O...

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Thank you for contacting us again and for providing some further details.

It may well be that the antibody has been accidentally damaged during shipment or transfer. I would suggest aliquotting this product and storing at -20°C or -80°...

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Thank you for contacting us. Please follow the procedure as given below; - Embed tissue in OCT medium. - Section it using cryostat - Place tissue sections on glass slide and dry the sections overnight at RT. - Fix the sections w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"